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Universal
Primer System At IGMC Can Save You Money!
Instead of
buying individually
fluorescently labeled oligos for genotyping, which can get expensive,
you can use unlabeled oligos along with a M13 labeled primer from the
center. The center will inexpensively provide fluorescently labeled
M13 primers which can be easily incorporated into most primer
systems. Users provide their individual unlabeled oligos with the
forward
primer containing the M13 sequence at the 5’, and use our labeled M13
primer to create labeled fragments for genotypic detection. This
method can easily be adapted to your existing PCR protocols with the
simple addition of a few more cycles to incorporate the M13 labeled
primer to your product. The center manager can help optimize PCR
protocols for use in this system. Contact the center for more
information. |
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